Extended Data Fig. 6: CCNT1’s Effect on HIV Transcription in Perturb-seq.
From: Decoding heterogeneous single-cell perturbation responses
a, The distribution of CCNT1 mixscape score, calculated by mixscape. b, The expressions of CCNT1 (left) and CCNT1-targeting gRNAs (right). c, Differential expression results between CCNT1-targeting cells and non-targeting control cells in two different cell states. Dotted horizonal line indicates the position corresponding to adjusted p-val=0.001. d, The expressions of NFKB1. e, The quantitative perturbation-expression relationship between GFP and CCNT1 PS, similar with Fig. 6e. The PCC p-values (calculated from Pearson’s test) are 0.37, 5.9e-6, and 0.04 for DMSO, GFP- and GFP+ conditions, respectively. f, CD69 mRNA expression correlates with the continuous, stimulated state of T cells. g-j, distribution of PS values in CD247 (g) and PDCD1 (i), and the cumulative distribution PS, grouped by CD69 expression quantiles (h,j). Wilcox test p values for CD247 is 1.26e-51 and for PDCD1 is 1.3e-3.
