Fig. 5: Perturb-seq on HIV latency.
From: Decoding heterogeneous single-cell perturbation responses
a, The experimental design of Perturb-seq. b, The UMAP plot of single-cell transcriptome profiles. Cells are coloured by three different conditions. c, The distribution of BRD4 PS. d, The expression of HIV-GFP. e, The correlations between HIV-GFP expression and BRD4 PS that does not use HIV-GFP as the target gene. The PCC P values (calculated from Pearson’s test) are 1.40 × 10−65, 1.96 × 10−6, 6.26 × 10−29; n = 457, 179, 402 for DMSO, GFP− and GFP+ conditions, respectively. The shaded area indicates a 95% confidence interval of locally estimated scatterplot smoothing regression. f, The distribution of CCNT1 PS. g, The protein expression of HIV-GFP in response to CCNT1 knockout in different cell states (TNF versus non-stimulated). Source numerical data are available in the source data.
