Extended Data Fig. 4: pEtn or TIFM treatment effects on CD8+ T cells can be reversed by Pcyt2-KO or pEtn addback, respectively.
From: Tumour interstitial fluid-enriched phosphoethanolamine suppresses T cell function
A-C) OT-I CD8 + T cells were activated and cultured as in Fig. 1a) in RPMI, TIFM, or TIFM formulated without pEtn and supplemented with RPMI levels of Arginine. (TIFM -pEtn +Arg) for 5 days. At the end of culture, cells were restimulated and analyzed for A) Granzyme B MFI B) IL-2 MFI and C) %IFNγ+TNF+ cells. Bar plots show quantified normalized MFI or %+ ±SEM on the left of each flow cytogram, data are from 3 independent experiments (n = 6 mice/group, p-values A) RPMI vs. TIFM p = 0.0014, TIFM vs. TIFM+Arg-pEtn p = 0.0458, RPMI vs. TIFM-pEtn+Arg p = 0.5720 B) RPMI vs. TIFM p = 0.0389, TIFM vs. TIFM+Arg-pEtn p = 0.0315 RPMI vs. TIFM-pEtn+Arg p = 0.9741 C) RPMI vs. TIFM p = 0.0019, TIFM vs. TIFM+Arg-pEtn p = 0.0210, RPMI vs. TIFM-pEtn+Arg p = 0.1116). Statistical analysis for all indicated comparisons in A-C) was determined using a one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. Source numerical data are available in source data.
