Extended Data Fig. 4: T cell development of Ldhbfl/flCd4-Cre mice.
a, IP and IB analysis of the interaction of G6PD protein with LDHB protein in WT exhausted CD8+ T cells. b, Signature score of T cell exhaustion-associated gene set in CRC and RCC-infiltrating LDHBhigh (CRC, n = 27262; RCC n = 6529) and LDHBlow (CRC, n = 22246; RCC n = 5396) CD8+ T cells. The boxes in a show the median ±1 quartile, with the whiskers extending from the hinge to the smallest or largest value within 1.5× the interquartile range from the box boundaries. c, RT-qPCR analysis of the expression levels of CD8+ T cell exhaustion signature genes in RCC-infiltrating CD8+ T cells (n = 7). The lowest expression level of LDHB in RCC-infiltrating CD8+ T cells was normalized to 1. LDHBhigh group, higher than 1.2; LDHBlow group, less than 1.2. d, IB analysis of LDHB protein level in Ldhb+/+Cd4-Cre and Ldhbfl/flCd4-Cre CD8+ T cells. e, Flow cytometric analysis of the percentage of CD4+ and CD8+ T cells in the thymus, spleen and lymph node of Ldhb+/+Cd4-Cre and Ldhbfl/flCd4-Cre mice (n = 3). f, Flow cytometric analysis of the percentage of tumor-infiltrating CD4+ T cells in tumors of Ldhb+/+Cd4-Cre and Ldhbfl/flCd4-Cre mice injected s.c. with MC38 colon cancer cells (day 16; IFN-γ+, n = 5; Foxp3+, n = 6; CXCR5+PD-1+, n = 7). Data were presented as mean ± SEM. P values were calculated by two-sided Wilcoxon rank-sum test (b) or two-tailed Student’s t test (a,c,e,f). ns, no statistically significance. In b, n indicates the number of CD8+ T cells. In c, n indicates the number of human samples. In e, n indicates the number of biological replicates. In f, n indicates the number of mice. For a, d-f, three independent experiments were performed.
