Extended Data Fig. 6: RNA-seq and ATAC-seq of exhausted Ldhbfl/flCd4-Cre CD8+ T cells.
a, Principal component analysis (PCA) of RNA-seq (upper panel) and ATAC-seq (lower panel) of exhausted Ldhb+/+Cd4-Cre and Ldhbfl/flCd4-Cre CD8+ T cells induced in vitro. b,c, Heatmap showing the differentially regulated gene expression (b) and peaks (c) among exhausted Ldhb+/+Cd4-Cre (WT) and Ldhbfl/flCd4-Cre (KO) CD8+ T cells (n = 3). d, qPCR analysis of the expression level of Ldhb in naïve (Tn) and exhausted (Tex) CD8+ T cells (n = 4). e, IB analysis of the protein level of LDHB in naïve (Tn) and exhausted (Tex) CD8+ T cells. Data were independently repeated 3 times (d,e). Data were presented as mean ± SEM and P values were calculated by two-tailed Student’s t test (d). In b-d, n indicates the number of biological replicates. For d, e, three independent experiments were performed.
