Extended Data Fig. 2: T cell activity of Slc7a11fl/+Cd8-Cre and Slc7a11fl/+Cd4-Cre T cells.
a, The percentage of PD-1+Tim-3+ and IFN-γ+ cells of CD8+ T cells in exhaustion-induced condition in vitro at day 2, 4, 6 and 8 (n = 4). b, Actin cytoskeleton protein in exhausted Slc7a11+/+Cd8-Cre (labelled as 1), Slc7a11fl/+Cd8-Cre (labelled as 2) and Slc7a11fl/flCd8-Cre (labelled as 3) CD8+ T cells at day 2, 4, 6 and 8. c, FLNA in exhausted Slc7a11+/+Cd8-Cre (WT), Slc7a11fl/+Cd8-Cre (Her) and Slc7a11fl/flCd8-Cre (KO) CD8+ T cells. d, Actin cytoskeleton protein in exhausted Slc7a11+/+Cd4-Cre (WT) and Slc7a11fl/+Cd4-Cre (Her) CD8+ T cells. e, Intracellular cystine level (n = 4) and PD-1+Tim-3+ cell percentage (n = 3) of Slc7a11+/+Cd4-Cre (WT) and Slc7a11fl/+Cd4-Cre (Her) CD8+ T cells in exhaustion induced condition in vitro. f, The percentage of TNF-ɑ+ and IFN-γ+ in Slc7a11+/+Cd4-Cre and Slc7a11fl/+Cd4-Cre splenic CD4+ and CD8+ T cells stimulated with anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) for 48 hours (n = 4). g, Tumor size of Slc7a11+/+Cd4-Cre (WT) and Slc7a11fl/+Cd4-Cre (Her) mice injected s.c. with 5 × 105 MC38 colon cancer cells (n = 6). h, The percentage of tumor-infiltrating PD-1+Tim-3+, TNF-ɑ+, IFN-γ+ CD8+ T cells (n = 6) and IFN-γ+ (n = 6), Foxp3+ (n = 7), CXCR5+PD-1+ (n = 6) CD4+ T cells in tumors of Slc7a11+/+Cd4-Cre and Slc7a11fl/+Cd4-Cre mice injected with MC38 cells (day 16). i, Tumor growth of Slc7a11+/+Cd4-Cre (WT) and Slc7a11fl/+Cd4-Cre (Her) mice injected with MC38 cells. These mice were treated (i.p.) with 200 mg anti-CD4 (ɑ-CD4) or control antibody (IgG) every three days for five times (n = 6). Data were presented as mean ± SEM. P values were calculated by two-tailed Student’s t test (e, f, h), one-way ANOVA with Dunnett’s multiple comparison (a) or two-way ANOVA with Geisser-Greenhouse correction (g, i); ns, no statistically significance. In a, e-f, n indicates the number of biological replicates. In g-i, n indicates the number of mice. For a-i, three independent experiments were performed.
