Extended Data Fig. 4: Characterization of YAP/TAZ-TDP-43 interaction.
From: YAP maintains the dynamics of TDP-43 condensates and antagonizes TDP-43 pathological aggregates
a,b, WT HeLa cells at baseline or treated with 500 µM NaAsO2 for 1 h were processed for immunostaining using the indicated antibodies. Pearson’s correlation between YAP and TDP-43 were quantified (b). The experiments were performed for a total of 2 independent times with similar results. 5 images for each condition from one representative experiment were analysed and plotted. Data were shown as mean ± s.d. c, HeLa cells with Flag–YAP KI treated with 500 µM NaAsO2 for 1 h were subjected to immunoprecipitation using Flag antibody, followed by immunoblotting analyses. The experiment was repeated two independently times with similar results. d, Pull-down assay using YAP WT and S94A immunopurified from 293T cells and recombinant His–MBP–TDP-43. Representative of 2 independent replicates. e, Cartoon illustrations of truncation mutants of YAP (left), TDP-43 (middle), and TAZ (right), respectively. f–i, The indicated truncations of YAP, TDP-43 or TAZ were used for pull-down assays. The experiments were repeated two independently times with similar results. j–l, The 2D 1H15N HSQC spectra of 50 µM 15N-labelled YAP TB domain titrated with TDP-43 at 1:1 molar ratio. The residues of YAP TB domain markedly diminished by TDP-43 titration were shown on the right (j). The corresponding profiles of the chemical shift and intensity ratio were shown in k and l, respectively.
