Extended Data Fig. 1: Many components of the Hippo pathway are localized to stress granules.
From: YAP maintains the dynamics of TDP-43 condensates and antagonizes TDP-43 pathological aggregates
a–d, Schematic illustration of Flag–MST1 (a), GFP–LATS1 (b), GFP–MOB1A (c), and Flag–YAP (d) KI in HeLa cells and screening by immunoblotting or PCR. e–i, Localization of Flag–MST1 (e), HA–SAV1 (f), GFP–LATS1 (g), GFP–MOB1A (h), Flag–YAP or TEAD1 (i) in HeLa cells treated with 500 µM NaAsO2 for 1 h. SGs were visualized with antibodies against either G3BP1 or TIAR. Pearson’s correlation and line graphs were shown next to the representative confocal images. 6 (e,g,h) and 8 (f,i) images were pooled from 2 independent experiments for each condition. Data were shown as mean ± s.d.
