Extended Data Fig. 3: Both YAP and TAZ are recruited into SGs and are non-redundant in promoting the disassembly of SGs.
From: YAP maintains the dynamics of TDP-43 condensates and antagonizes TDP-43 pathological aggregates
a–d, WT HeLa cells treated with NaAsO2 (500 µM, 1 h), H2O2 (800 µM, 1 h), MG132 (40 µM, 3 h) or heat shock (43 °C for 1 h) were processed for immunostaining using antibodies against endogenous YAP and G3BP1 (a) or TAZ and G3BP1 (c), respectively. Pearson’s correlation between YAP and G3BP1 (b) or TAZ and G3BP1 (d) was calculated, respectively. 8 images were pooled from 2 independent experiments for each condition. mean ± s.d. e–h, HeLa cells transfected with the indicated Flag-tagged truncations of YAP (e) or TAZ (g) were treated with NaAsO2 for 1 h and then immunostained with antibodies against Flag and G3BP1. Pearson’s correlation between YAP truncations and G3BP1 (f), or TAZ truncations and G3BP1 (h) was calculated, respectively. 8 images were pooled from 2 independent experiments for each condition. mean ± s.d. i, WT HeLa cells transfected with the indicated siRNA were analysed by immunoblotting with the indicated antibodies. Representative of 2 independent replicates. j,k, WT HCT116 or U-2 OS cells transfected with siCtrl, siYAP, siTAZ, or siYAP/TAZ were treated with 500 µM NaAsO2 for 40 min, then allowed to recover in fresh media for 2 h. The percentage of cells with SGs was quantified for each cell type in j, or k, respectively. j, The number of cells per condition are 759 (baseline siCtrl), 517 (baseline siYAP), 507 (baseline siTAZ), 923 (baseline siYAP/TAZ), 759 (NaAsO2 siCtrl), 477 (NaAsO2 siYAP), 457 (NaAsO2 siTAZ), 881 (NaAsO2 siYAP/TAZ), 773 (recovery siCtrl), 467 (recovery siYAP), 475 (recovery siTAZ), 979 (recovery siYAP/TAZ), respectively, pooled from n = 3 independent experiments. k, The number of cells per condition are 686 (baseline siCtrl), 828 (baseline siYAP), 767 (baseline siTAZ), 735 (baseline siYAP/TAZ), 748 (NaAsO2 siCtrl), 712 (NaAsO2 siYAP), 720 (NaAsO2 siTAZ), 678 (NaAsO2 siYAP/TAZ), 744 (recovery siCtrl), 680 (recovery siYAP), 691 (recovery siTAZ), 669 (recovery siYAP/TAZ), respectively, pooled from n = 3 independent experiments. mean ± s.e.m. one-way ANOVA followed by Dunnett’s test (j,k).
