Extended Data Fig. 2: Additional data for Fig. 2. | Nature Cell Biology

Extended Data Fig. 2: Additional data for Fig. 2.

From: Two microbiome metabolites compete for tRNA modification to impact mammalian cell proliferation and translation quality control

Extended Data Fig. 2

ns: not significant, * p < 0.05, ** p < 0.01, *** p < 10−3. Panel (a, c): Mann-Whitney U test, two sided. Panel (e): Tukey’s Honestly Significant Difference test (HSD), two-sided. Data are presented as mean values ± SD. a) Proliferation measurement by absorbance at 460 nm, shRNA-QTRT1 knockdown, and control HEK293T cells. Arrow indicates the time of addition of preQ1 and/or queuine. Error bar corresponds to the range of n = 8 biological replicates. b) Western blot showing shRNA knockdown of the QTRT2 protein. CycB is the loading control. c) Same setup as panel (a), except for shRNA-QTRT2 and control cells. d) Western blot of QTRT1 protein in HEK293T, MEF, and BMDC cells. Vinculin is the loading control. e) Quantification of QTRT1 level in panel (d). n = 3 biological replicates. p values from left to right: 0.097, 8.0e-4, 0.007. f) Reaction scheme of the preQ1-modified tRNA with NHS ester for Northern blot analysis. Source numerical data and unprocessed blots are available in.

Source data

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