Extended Data Fig. 3: Additional data for Fig. 3. | Nature Cell Biology

Extended Data Fig. 3: Additional data for Fig. 3.

From: Two microbiome metabolites compete for tRNA modification to impact mammalian cell proliferation and translation quality control

Extended Data Fig. 3

ns: not significant, * p < 0.05, ** p < 0.01, *** p < 10−3, **** p < 10−4. Mann-Whitney U test, two sided. Data are presented as mean values ± SD. a) LC-MS/MS of mouse feces showing queuine and preQ1 metabolites and queuosine, and preQ1sine nucleosides. The Y axis represents the concentrations of preQ1, queuine, queuosine and preQ1sine. Mean values on top of graph. n = 2 biological replicates. b) Quantitation of Q-modification levels from Northern blot results of preQ1 injected liver and kidney tRNAHis and tRNAAsn. n = 4 biological replicates. p values from left to right: 0.0152, 0.0152, 0.0152, 0.0152. c) Proliferation measurement by absorbance at 460 nm, B16 cells. Arrow indicates the time of addition of preQ1 and/or queuine. Error bar corresponds to the range of n = 8 biological replicates. Source numerical data are available in.

Source data

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