Extended Data Fig. 7: Niche-derived KITL promotes melanomagenesis.
From: Antagonistic stem cell fates under stress govern decisions between hair greying and melanoma
a, Immunohistological analysis of the distribution of GFP+ melanocytes in the epidermis of WT and of Kitl cHetero mice at 4 weeks post-onset DMBA treatment. GFP+ melanocytic lineages are visualized with Dct-H2B-GFP KI mice. Arrows indicate melanocytes in the IFE. Graph showing the number of GFP+ cells in the epidermis (WT; n=5, Kitl cHetero; n=4, biological independent experiments). The experimental scheme for DMBA treatment is shown in Extended Data Fig. 2a. Scale Bar = 100 μm. Two-tailed Student’s t-test. b, Macroscopic views of the dorsal skin of WT and of Kitl cHetero mice at 16 weeks post-onset DMBA treatment. Graph showing the number of melanocytic pre-cancerous lesions (indicated by arrowheads) on the dorsal skin (WT; n=8, Kitl cHetero; n=5, biological independent experiments). The experimental scheme for DMBA treatment is shown in Extended Data Fig. 2a. Two tailed Student’s t-test. c, Line graph showing the number of melanocytic precancerous lesions on the dorsal skin at the indicated time points after DMBA treatment (Ink4a/Arf−/−; K14-Kitl; n=3, Ink4a/Arf−/−; n=3, Ink4a/Arf+/−; K14-Kitl; n=12, Ink4a/Arf+/−; n=6, Ink4a/Arf+/+; K14-Kitl; n=5, Ink4a/Arf+/+; n=9, biological independent experiments). Data are presented as means ± SEM (a-c). d, Macroscopic view of dorsal skin showing melanocytic tumors (arrow) in Mc1re/e; Ink4a/Arf+/−; K14-Kitl mice at the indicated time points.
