Extended Data Fig. 9: Decreased expression of niche-derived cytokines and down-regulation of the p53 pathway in an aged niche for McSCs.
From: Antagonistic stem cell fates under stress govern decisions between hair greying and melanoma
a, Graphs showing the expression of the indicated genes in FACS-purified HFSCs/Hg at early anagen (n=3, biological independent experiments). Two-tailed Student’s t-tests. b,c, Pathways down-regulated (b) or up-regulated (c) in FACS-purified HFSCs/Hg at early anagen analyzed by GSEA using the c2.cp.biocarta.v7.5 gene set collection (permutation test). d, Enrichment plot for Biocarta P53_pathway (Young, early anagen vs Old, early anagen). e, Heatmap showing the Biocarta P53_pathway gene set core signature. Colors range from dark red to dark blue representing the highest and lowest expression of each gene, respectively. f, Graphs showing the expression of p53, Cdkn1a (p21) and Cdkn2a (p16) in HFSCs/Hg at telogen and early anagen (n=3, biological independent experiments). Two-tailed Student’s t-tests. g, Enrichment plot for KEGG P53_signaling_pathway (Vehicle, early anagen vs DMBA, early anagen). Data are presented as means ± SEM (a,f).
