Extended Data Fig. 5: Comparison of cationic lipid/siRNA/polymer hybrid NPs stabilized by digital lipid versus that by DSPE-PEG. | Nature Chemistry

Extended Data Fig. 5: Comparison of cationic lipid/siRNA/polymer hybrid NPs stabilized by digital lipid versus that by DSPE-PEG.

From: Digital micelles of encoded polymeric amphiphiles for direct sequence reading and ex vivo label-free quantification

Extended Data Fig. 5

a,b, Fluorescence emission spectra recorded for two types of FAM-siRNA-Luc hybrid NP dispersions and corresponding supernatant solutions after initially formed hybrid NPs were subjected ultrafiltration (MWCO, 100 kDa). c, Evolution of DLS sizes upon coincubation with 20% FBS. d,e, Evolution of emission intensities of two types of FAM-siRNA-Luc hybrid NPs upon co-incubation with 20% FBS. f, Uptake extents of FAM-siRNA-Luc hybrid NP stabilized by the digital lipid when co-incubated with BXPC-3 cells, as quantified by MALDI–TOF MS in a label-free manner (n = 3 biologically independent cells; data are presented as mean values ± SEM). g, CLSM images (FAM channel) of BXPC-3 cells upon co-incubation with two types of FAM-siRNA-Luc hybrid NPs, whereas naked FAM-siRNA-Luc was used as a control. Scale bar: 25 μm.

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