Extended Data Fig. 2: Biochemical analysis of Tri17-mediated dehydration of 18.
From: Enzymatic synthesis of azide by a promiscuous N-nitrosylase
A Tri17 biochemical assay with 17 was incubated at room temperature for 30 minutes and the protein was removed immediately using an Amicon spin filter (2 kDa MWCO). The reaction flowthrough was extracted with ethyl acetate, dried, and served as substrates (containing a mixture of 17, 18, and 19) for new Tri17 reactions and the production of 19 was monitored. The EICs demonstrate increased production of 19 in a Tri17-dependent manner. A 10-ppm error mass tolerance was used for each trace. The data points and error bars present in the bar graph represent the average and standard deviations of 19 produced from three independently performed experiments.
