Extended Data Fig. 1: Cofactor preference of Abx(−)E and characteristics of products of Abx(−)E. | Nature Chemistry

Extended Data Fig. 1: Cofactor preference of Abx(−)E and characteristics of products of Abx(−)E.

From: An enzymatic dual-oxa Diels–Alder reaction constructs the oxygen-bridged tricyclic acetal unit of (–)-anthrabenzoxocinone

Extended Data Fig. 1

a, Cofactor preference of Abx(−)E. (i) standard (+)-ABX; (ii) standard (−)-ABX; (iii) 100 μM 1 in 80 μM Abx(−)E; (iv) 100 μM 1 in 80 μM Abx(−)E and 1 mM NADPH. (v) 100 μM 1 in 80 μM Abx(−)E and 1 mM NADH; (vi) 100 μM 1 in 80 μM Abx(−)E, 5 μM GDH, 20 mM glucose. There was no addition of NADPH to the reaction system, and the activity detected implies that the endogenous cofactor (*NADP+) was present in Abx(−)E. b, Time-course conversion of 100 μM 1 in the presence of 80 μM Abx(−)E which was analyzed by normal UPLC (λ = 365 nm). The 8 multiplier mean the heights of the tiny peaks corresponding to compound 5 are magnified eight times for a better visibility. c, UV spectra and mass of compound 2, 5 and (±)-ABX.

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