Extended Data Fig. 2: Mutations in strains carrying a single mutant copy of the TDH3 promoter.
(a) Schematic shows the reference sequence and mutations tested in the RAP1 binding site (yellow), two GCR1 binding sites (blue, red), and the TATA box (gray) of the TDH3 promoter. Coordinates shown are the number of bases upstream of the start codon of the downstream YFP or TDH3 protein. (b) The mutation(s) present in each of the 34 mutant PTDH3 alleles assayed with a single copy of the PTDH3-YFP reporter gene are shown. In each case, the first strain listed (YPW####) carries the mutant TDH3 promoter allele cloned upstream of a YFP reporter protein at the HO locus and was used to measure the effects of the mutation(s) on gene expression whereas the second strain listed carries the mutant PTDH3 allele at the native TDH3 locus and was used to measure the effects of the mutation(s) on fitness (Supplementary Table 1). Asterisks show alleles that were used to compare environment-dependent effects of disrupting TATA box or the TFBS.
