Extended Data Fig. 3: Mutations in strains carrying two mutant copies of the TDH3 promoter.

The schematic at the top of the figure shows the arrangement of two copies of the TDH3 promoter driving expression of either a YFP reporter protein at the HO locus or the native TDH3 protein at the native locus, with each gene pair separated by a copy of the URA3 gene. The specific mutations carried in each of the 13 pairs of mutant strains carrying two copies of the P_TDH3 allele are then shown with the name (YPW####) of the strain carrying the reporter genes used to measure effects on gene expression listed before the name of the strain with the duplication of TDH3 used to measure fitness (Supplementary Table 1). For each pair of strains, the mutated elements are underlined and the sequences of these elements are shown for each copy of the TDH3 promoter with the mutated sites shown in red. The color of the underline corresponds to the feature mutated: RAP1 binding site = yellow, GCR1a binding site = blue, GCR1b binding site = red, and TATA box = gray.