Extended Data Fig. 4: Functional studies of different ABCGpt genes in programmed body colour transition.
From: The pigment transporter Redboy confers programmed body colour transition in orchid mantises
a, Effect of repression different ABCG pigment transporter (ABCGpt) genes on the fading of red pigments during the first moult. b, Relative expression of different ABCGpt genes in the abdominal cuticle (AC), fat body (FB), head, Malpighian tubule (MT), gut, leg, and thorax of N2D0 mantises. Data are shown as mean ± SEM, different letters indicate statistically significant differences between groups using Welch’s ANOVA (Games–Howell multiple comparisons test, P < 0.05, n = 4 biological replicates, individuals are represented by dots), all statistical tests are two-tailed. c, Fluorescence in situ hybridization of Redboy in the integument of early fourth-instar nymphs. The four diagrams in the upper left corner represent DAPI (blue), FITC (green), bright field and superposition diagram respectively. The four diagrams showcases in the lower left corner correspond to enlarged pictures. The green fluorescence signal in the dsMuslta sample disappeared in the right Redboy-RNAi samples, suggesting that the green signal represents Redboy mRNA. Notably, the signal in the epidermis is a non-specific signal of chitin in the cuticle. d, DX injected into the haemolymph is normally excreted into the MT after the knock-down of Brown. e, Inhibition of Brown or White causes the yellow pigment in the MT to disappear. f, Influence of inhibition different ABCGpt genes using the second RNAi targets on the body color of fourth-instar nymphs. Source data are provided in Source Data Extended Data Fig. 4.
