Extended Data Fig. 8: Growth rates along evolutionary trajectories in the absence of antibiotic.
From: Cryptic phenotypic variation emerges rapidly during the adaptive evolution of a carbapenemase
(a) Optical density changes over 12 h of growth. The black line represents the strain with wild-type KPC-2, thick colored lines represent the first round strains, and the thiner colored lines represent plateau strains. (b) Boxplot summarizing maximum growth rates from the growth curves above. X-axis labels, excluding the control strain, indicate the evolutionary line (as in Table S2) and the round from which each isolate was taken (that is, rounds 1–4, labeled R1–R4). Following R’s default convention, boxes span the first (Q1) to third (Q3) quartiles, central lines mark the median, and whiskers reach the most extreme points within 1.5 times the interquartile range. (c) Growth rates in the absence of antibiotic show a moderate negative correlation with those measured in the presence of subinhibitory concentrations of CAZ, albeit non-significant (R = –0.65, P = 0.11). Solid line indicates the ideal one-to-one correspondence.
