Extended Data Fig. 4: Western blot of KPC-2 and its variants in periplasmic extracts of E. coli TOP10.
From: Cryptic phenotypic variation emerges rapidly during the adaptive evolution of a carbapenemase
(a–d) Periplasmic abundance of representative lineages from groups H274 (a, green), L169 (b, yellow), D179 (c, blue), and S171 (d, red) after 1 and 5 h of growth. (e) Immunoblot of selected KPC variants in the periplasm of E. coli TOP10 after 10 h of incubation. Isolates from each lineage are identified by the top label and color-coded as in previous panels. The bottom label indicates the evolutionary round of each isolate. Note that for groups D1 & D2 and S1 & S2, the earliest isolate shown (R2 and R1, respectively) is ancestral to both descendant lineages. Repeated round labels (R3 and R4, respectively) represent isolates sampled after lineage splitting and are arranged in natural alphanumeric order (for example, round 3 D1, round 3 D2).
