Extended Data Fig. 8: Impact of vsp1 knockdown on neural crest cells motility and chemotaxis ex vivo.
From: Stretch-induced endogenous electric fields drive directed collective cell migration in vivo
a, Overlay of isolated neural crest cells at 0 min (t1) and 60 min (t2) of migration. White lines depict cell trajectories. Scale bar, 30 μm. b, Quantification of single cells normalized velocity. Red lines represent mean and error bars standard deviation. One-way ANOVA with Dunnett's multiple comparisons test, nControl = 14, nXl-Vsp1-C301S = 19, nVsp1-SSMO = 9 and nVsp1-Cas13 = 9 cells. c, Scheme of a chemotaxis assay. d, Top panels show clusters after 5 min of migration towards SDF-1 and bottom panels show clusters arriving to the SDF-1 beads after 3–4 h (red arrows depict the direction of migration). Scale bar, 100 μm. e, Forward migration index (FMI) quantifications. Red lines represent mean and error bars standard deviation. One-way ANOVA with Dunnett's multiple comparisons test, nControl = 14, nXl-Vsp1-C301S = 9, nVsp1-SSMO = 7 and nVsp1-Cas13 = 8 clusters. a,d, Representative examples from three independent experiments; CI = 95%.
