Fig. 3: Vsp1 is required for neural crest directional response to electric fields.
From: Stretch-induced endogenous electric fields drive directed collective cell migration in vivo
a, Cartoon depicting a typical electrotaxis experiment. b, Time-colour-coded cluster trajectories ex vivo, showing the impact of Vsp1 knockdowns in the migration of neural crest clusters (the conditions are indicated). The arrows indicate the direction of migration. Scale bar, 100 μm. c, FMI. The red lines represent the mean and the error bars, the standard deviation. Two-tailed Student’s t-test, ****PXl-Vsp1-C301S < 0.0001 (with Welch’s correction), ****PVsp1-SSMO < 0.0001 and ****PVsp1-Cas13 < 0.0001 (with Welch’s correction), nControl = 26, nXl-Vsp1-C301S = 20, nControl = 28, nVsp1-SSMO = 33, nControl Cas13 = 35 and nVsp1-Cas13 = 33 clusters. d, Neural crest graft assays. e, Time-colour-coded neural crest trajectories in vivo (lateral view). Scale bar, 200 μm. f, FMI quantifications. The red lines represent the median and the error bars, the interquartile ranges. Two-tailed Mann–Whitney U-test, ****P < 0.0001 (for all comparisons), nControl = 29, nXl-Vsp1-C301S = 43, nControl = 26 and nVsp1-SSMO = 39 cells, nControl = 36 and nVsp1-Cas13 = 34 cells. b,e, Representative examples from at least three independent experiments; CI = 95%.
