Extended Data Fig. 4: Electrotactic response of neural crest clusters in a physiological range of electric field strength.
From: Stretch-induced endogenous electric fields drive directed collective cell migration in vivo
a, Time colour-coded trajectories of neural crest clusters migrating under the indicated conditions. Arrows indicate trajectories. Scale bar, 100 μm. Representative examples from three independent experiments; CI = 95%. b–d, Quantification of migratory parameters. Red lines represent median and error bars interquartile range. nNo EF = 27, n10 mV mm−1 = 32, n50 mV mm−1 = 18, n100 mV mm−1 = 44 clusters. b, Forward migration index (FMI). No EF vs. 10 mV mm−1, two-tailed t-test with Welch’s correction, *p = 0.0253; No EF vs. 50 mV mm−1, two-tailed t-test, ****p < 0.0001; No EF vs. 100 mV mm−1, two-tailed t-test with Welch’s correction, ****p < 0.0001; 10 vs. 50 mV mm−1, two-tailed t-test, ****p < 0.0001; 50 vs. 100 mV mm−1’, two-tailed t-test with Welch’s correction, *p = 0.0436. c, Velocity. No EF vs. 10 mV mm−1, two-tailed t-test, p = 0.0967; No EF vs. 50 mV mm−1, two-tailed t-test, ***p = 0.0005; No EF vs. 100 mV mm−1, two-tailed Mann Whitney U-test, ****p < 0.0001; 10 vs. 50 mV mm−1, two-tailed t-test, **p = 0.0065; 50 vs. 100 mV mm−1, two-tailed Mann Whitney U-test, *p = 0.0212. d, Net displacement. No EF vs. 10 mV mm−1, two-tailed t-test, *p = 0.0201; No EF vs. 50 mV mm−1, two-tailed t-test, ****p < 0.0001; No EF vs. 100 mV mm−1, two-tailed Mann Whitney U-test, ****p < 0.0001; 10 vs. 50 mV mm−1, two-tailed t-test; **p = 0.0010; 50 vs. 100 mV mm−1, two-tailed Mann Whitney U-test, *p = 0.0171.
