Extended Data Fig. 4: Characterization of AT2 and AT1 markers in control PCLS.

a. Representative fluorescent images of LAMP3 and PDPN staining in CTRL murine PCLS over a 5-day culture period (scale bar 100 μm). b. Quantification of AT2-specific marker lysosomal associated membrane protein 3 (LAMP3) positive cells in murine PCLS up to day 5 day after PCLS preparation (n = 10 images per timepoint from one representative mouse, *p=0.0195, ns = not significant by Kruskal-Wallis test with Dunn’s multiple comparisons). For all box plots, the centre line represents the median, the box limits are upper and lower quartiles, and the whisker limits are the minimum and maximum points on the plots.