Extended Data Fig. 5: Cell motility in response to tissue stiffening.
a. Representative live SftpcGFP cell fluorescent images of lineage-traced cells in CTRL and STIFF regions of murine PCLS for up to 48 hours (white arrows for representative cells, scale bar 100 µm) with respective migration and rosette plots over 48 hours and analyses of displacement and directionality ratio (n = 60 cells per group, **p=0.0033, ****p<0.001 by two-tailed unpaired Mann Whitney test). b. Quantification of average 3T3 cell speed with (+) versus without (-) blue light exposure over 1 hour (n = 14 images of 3 independent experiments, ns = not significant by unpaired Student’s t-test with Welch’s correction). c. Representative fluorescent images and quantification of normalized mean dityrosine immunofluorescence in CTRL and STIFF regions of embedded mouse lung fibroblasts in hydrogels (n = 8 images per group (CTRL, STIFF) from one representative hydrogel, ns = not significant by two-tailed unpaired Mann Whitney test, scale bar 50 µm). For all box plots, the centre line represents the median, the box limits are upper and lower quartiles, and the whisker limits are the minimum and maximum points on the plots.
