Extended Data Fig. 2: The effect of infection dose on the potency of ex vivo CD4+ T cell-mediated responses. | Nature Microbiology

Extended Data Fig. 2: The effect of infection dose on the potency of ex vivo CD4+ T cell-mediated responses.

From: Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella

Extended Data Fig. 2

Wild-type mice were challenged with either 1.58 × 105 CFU, 1.72 × 104 CFU or 1.53 × 103 CFU S. Typhimurium M525P. Mice were euthanised 104-125 days after challenge and their splenic CD4+ T cells were stimulated with medium only (w/o stim.), anti-mouse CD3e (anti-CD3e) or whole bacterial cell extract (extract). a, IFN-γ measured by ELISA in the cell culture supernatant after 72 hours. b, IL-2 measured by ELISA in the cell culture supernatant after 24 hours. Data are shown as mean ± s.e.m. Statistical significance was calculated by a one-way ANOVA. Data were generated from one experiment.

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