Extended Data Fig. 4: Putative toxin gene expression is cell-, time- and oxygen-dependent.
From: Mucoricin is a ricin-like toxin that is critical for the pathogenesis of mucormycosis
a, Toxin gene expression in R. delemar germinating cells in YPD medium. Data (n = 3 wells/timepoint, pooled from three independent experiments) are presented as median ± interquartile range. Statistical analysis was performed by using unpaired t-test (two-tailed). b, Confocal imaging of Alexa Flour 488-labelled IgG anti-toxin (green) during the growth of R. delemar from spores to hyphae. Scale bar is 50 µm. c, Toxin gene expression from R. delemar hyphae grown in YPD culture in sufficient versus limited oxygen (n = 6 wells, data pooled from three independent experiments). Data presented as median ± interquartile range. Statistical analysis was performed by using unpaired t-test (two-tailed). d, Toxin gene expression analysis of fungal germlings on different cell types showed a time dependent expression on alveolar epithelial cells compared to HUVECs and erythrocytes (n = 3 wells/group, pooled from three independent experiments). Data presented as median ± interquartile range. Statistical analysis was performed by using unpaired t-test (two-tailed).
