Extended Data Fig. 5: RNAi targeting the putative R. delemar toxin inhibits its expression.
From: Mucoricin is a ricin-like toxin that is critical for the pathogenesis of mucormycosis
a, R. delemar spores were transformed with RNAi plasmids targeting the putative toxin (RNAi-toxin) or empty plasmid (Empty-plasmid) using biolistic delivery system. Cells were grown in minimal medium without uracil for 24 h prior to extracting RNA (n = 6/group, pooled from three independent experiments). Data presented as median ± interquartile range. Statistical analysis was performed by using Mann-Whitney non-parametric (two-tailed) test comparing RNAi- R. delemar toxin vs wild-type or empty plasmid b, Representative western blot and densitometry analyses of the wild-type, empty plasmid, or RNAi toxin strains (n = 4 pictures data pooled from four independent experiments) Data presented as median ± interquartile range. Statistical analysis was performed by using Mann-Whitney non-parametric (two-tailed) test comparing RNAi- R. delemar toxin vs. wild-type or empty plasmid. c, confocal images showing reduced expression of the toxin in the RNAi toxin mutant. Scale bar is 50 µm.
