Extended Data Fig. 4: The occurrence of IA91 growth in cultures supplemented with peptidoglycan-derived compounds.4.
From: A Marine Group A isolate relies on other growing bacteria for cell wall formation
The final concentration of each compound in the culture medium was 10 µg ml−1. IA91 growth was determined by CH4 production after one month of cultivation and microscopy after 10 days of cultivation. IA91 was cultured with H2-utilizing Methanothermobacter thermautotrophicus strain ΔH. For CH4 production, means ± standard deviation of triplicate cultures are shown (a). CH4 detection limit was 0.5 mM. Representative phase-contrast micrographs are shown (b-i). SYBR Green I was used to stain DNA (b-i). Cells of co-cultured M. thermautotrophicus strain ∆H are visible in cyan due to their F420 autofluorescence. n=3 independent experiments. Abbreviation; PG, peptidoglycan; MP, muropeptide; MurNAc, N-acetylmuramic acid; GlcNAc, N-acetylglucosamine; AA, PG amino acids (that is, D-Ala, D-Glu and L-Lys). (Scale bars: 5 µm, b-i).
