Extended Data Fig. 5: SFTSV induces ferroptosis in THP-1 cells and human PBMCs.
From: Taurolithocholic acid protects against viral haemorrhagic fever via inhibition of ferroptosis
a,b, Levels of intracellular Fe2+ detected in SFTSV-infected THP-1 cells (a; n = 3 biologically independent samples) and hPBMCs (b; n = 6 biologically independent samples). c, Gating strategies for Fe2+ detection presented on Fig. 4a, m, Extended Data Figs. 5a, b and 8a. d, e, Levels of lipid ROS detected in SFTSV-infected THP-1 cells (d; n = 3 biologically independent samples) and hPBMCs (e; n = 6 biologically independent samples). f, Gating strategies for lipid ROS detection presented on Figs. 4c, n, p, 5a, c, e, g, i, k, l, Extended Data Figs. 5d, e, s–v, 7c, d, f, g and 8c. g–j, The mRNA levels of ferroptosis and iron metabolism related genes analyzed using RNA-seq data from THP-1 cells; n = 4 biologically independent samples. k,l, The mRNA levels of ferroptosis and iron metabolism related genes measured in THP-1 cells (k) and hPBMCs (l); n = 3 biologically independent samples. m,n, Lelves of mtROS detected in THP-1 cells (m; n = 3 biologically independent samples) and hPBMCs (n; n = 6 biologically independent samples) post infection with SFTSV (MOI = 1). o, Gating strategies for mitochondrial staining presented on Fig. 4i, j, l, and Extended Data Fig. 5m, n, p, q. p, q, The mitochondrial mass measured in THP-1 cells (p, n = 3 biologically independent samples) and hPBMCs (q, n = 6 biologically independent samples) post infection with SFTSV (MOI = 1). r, Gating strategies for mitochondrial mass detection presented on Fig. 4k. The change in fluorescence between (2) and (1) indicates the continuous activation of mitochondrial permeability transition pores. s,t, SFTSV-infected THP-1 cells (s, n = 3 biologically independent biologically independent samples) and hPBMCs (t, n = 6 biologically independent biologically independent samples) were incubated with Lip-1 (4 μM) or DFO (25 μM) for 72 h, and the lipid ROS was determined as above. u,v, SFTSV-infected THP-1 cells (u) and hPBMCs (v) were incubated with rotenone (10 nM) for 72 h, and the lipid ROS was determined as above; n = 3 biologically independent samples. Data were presented as mean ± s.d. The two-sided p values were examined using One-way ANOVA followed by Tukey’s multiple comparisons test for comparison of continuous variables among multiple groups (a,b,d,e,k,l,s-v), or Student’s t test for comparison of variables between two groups (g-j,m,n,p,q).
