Extended Data Fig. 2: Gating strategy for MIg-Seq protocol. | Nature Microbiology

Extended Data Fig. 2: Gating strategy for MIg-Seq protocol.

From: Metagenomic immunoglobulin sequencing reveals IgA coating of microbial strains in the healthy human gut

Extended Data Fig. 2

The preliminary gate to identify IgA-positive cells was SSC and SYBR Green, with SYBR-positive gate drawn based on preliminary experiments using a sample without SYBR green (SYBR stains all bacteria). SYBR-positive cells were then analyzed for IgA staining, with an IgA-positive gate drawn based on a sample without IgA staining. Representative flow plots are shown for a preliminary experiment of a single sample (#1037) that is unstained and without SYBR (A), staining and unsorted (B), and stained and sorted (C).

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