Extended Data Fig. 3: MIg-Seq assay antibody binding validation. | Nature Microbiology

Extended Data Fig. 3: MIg-Seq assay antibody binding validation.

From: Metagenomic immunoglobulin sequencing reveals IgA coating of microbial strains in the healthy human gut

Extended Data Fig. 3

A) Schematic of assay design for multistep magnet cell sorting, highlighting the motivation of using irrelevant magnetic beads to assess for non-specific bacterial binding to the magnetic beads. B) Stool was stained with Anti-Human IgA APC antibody and then with either anti-APC + magnetic beads as an experimental condition or anti-PE + magnetic beads as an irrelevant control prior to magnetic enrichment and analysis of the positive fraction via flow cytometry. C) DNA concentration in the positive fractions was obtained using Qubit™ dsDNA Quantification Assay Kit.

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