Extended Data Fig. 6: HCC patient fecal microbiota impairs gut barrier function and promotes bacteria translocation into liver in SPF mice with or without DEN treatment as shown in Fig. 1f and Extended Data Fig. 3a.
(a) Gut permeability assay using 500 kDa FITC-dextran. n=6 biologically independent samples. (b) Representative pictures of Alcian blue staining, E-Cad, CLDN 3 IHC staining, and Cy3-conjugated EUB338 probe FISH of colon tissues with quantitative analysis. n=3 biologically independent samples. (c) Representative bacterial culture of liver tissues under anaerobic and aerobic conditions with quantitative analysis. n= 19 (SPF/PBS), 16 (SPF/HD-FMT), 16 (SPF/HCC-FMT), 20 (SPFD/PBS), 16 (SPFD/HD-FMT), 16 (SPFD/LC-FMT), 16 (SPFD/HCC-FMT). From left to right and top to bottom, the culture plates are blood agar plate, chocolate blood agar plate, MacConkey agar plate, and Columbia blood agar plate, respectively. And, representative images of Cy3-conjugated EUB338 probe FISH detection in mice liver tissues. n=6 biologically independent samples. Data (excluding liver with live bacteria) are presented as mean ± SEM. Each data point in bar plots represents one mouse. Liver with live bacteria was calculated using Fisher’s exact test. Unless otherwise stated, statistical significance was calculated using one-way ANOVA. Adjustments were made for multiple comparisons.
