Extended Data Fig. 8: K. pneumoniae initiates gut barrier dysfunction through elevating colon macrophage mediated MMP-2/-9 activities and promotes precancerous lesions in germ-free mice.
(a) Representative pictures of TEM, Alcian blue staining, E-Cad, CLDN 3, CLDN 1 IHC staining, and Cy3-conjugated EUB338 probe FISH of colon tissues related to Fig. 4c. (b) Macrophage abundance in colon was evaluated by flow cytometry related to Fig. 4e. n = 6 biologically independent samples. (c) Cy3-conjugated conjugated EUB338 probe (PBS group), K. pneumoniae probe (KP and KPC groups) FISH detection in liver tissue related to Fig. 4f. n = 6 biologically independent samples. (d) Representative pictures of TEM, Alcian blue staining, E-Cad, CLDN 1 IHC staining, and Cy3-conjugated conjugated EUB338 probe (PBS group), K. pneumoniae probe (KP and KPC groups) FISH detection of colon tissues with quantitative analysis related to Fig. 4h. (e) Representative pictures of TEM and E-Cad IHC staining of liver tissues related to Fig. 5c. Masson’s trichrome staining and COL IV IHC staining of colon tissues related to Fig. 5e. (f) Representative pictures of PCNA and Ki-67 IHC staining related to Fig. 5g. (g) Representative pictures of Desmin and α-SMA IHC staining, and Sirius red staining of liver sections related to Fig. 5h. (h) Mouse Cancer Pathway Finder Array and Mouse Inflammatory Response and Autoimmunity PCR Array of liver tissues related to Fig. 5a. n = 3 independent experiments with similar results. Data (excluding PCR array results) are presented as mean ± SEM. Each data point in bar plots represents one mouse. Statistical significance was calculated using one-way ANOVA. Adjustments were made for multiple comparisons.
