Extended Data Fig. 7: MD analysis of HD5-P2Y11 interaction and distribution of P2Y11 in HeLa cells and human colonic samples. | Nature Microbiology

Extended Data Fig. 7: MD analysis of HD5-P2Y11 interaction and distribution of P2Y11 in HeLa cells and human colonic samples.

From: Shigella infection is facilitated by interaction of human enteric α-defensin 5 with colonic epithelial receptor P2Y11

Extended Data Fig. 7

a, Root-mean-square deviation variance analysis of the steady engagement of HD5 with P2Y11 after 50 ns in the 100-ns dynamic trajectory. b, Fluorescence microscopy analysis of P2Y11 distribution in the absence or presence of 2 μM HD5 in HeLa cells. P2Y11 is green, F-actin is red and nuclei are blue (DAPI). Cell outlines are indicated by the dash lines and arrows. The bar represents 10 μm. c, Fluorescence microscopy analysis of P2Y11 distribution in two human colonic tissue (samples 2 and 3). P2Y11 is green, F-actin is red, and nuclei are blue (DAPI). The bar represents 100 μm. The right panels show gray values of P2Y11 signals in the apical and basolateral regions in human colonic crypt in the samples. All results are representative of at least three independent experiments.

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