Extended Data Fig. 4: PTD-mediated proteasomal degradation of viral M1 protein of PROTAR viruses is dependent on their respective E3 ubiquitin ligase.
Conventional cells were transfected with indicated small interfering RNAs (siRNAs) (20 nM) or short hairpin RNAs (shRNAs) (1 µg/mL) for knockdown of each of the E3 ubiquitin ligases or scrambled RNA control (Ctrl RNA), and 24 h later infected with indicated viruses (MOI = 0.1). 48 h after infection, cells were collected for detection of the levels of influenza M1 protein by western blotting (n = 3).
