Extended Data Fig. 5: PTD-mediated proteasomal degradation of viral M1 protein of PROTAR viruses is E3 ubiquitin ligase-dependent. | Nature Microbiology

Extended Data Fig. 5: PTD-mediated proteasomal degradation of viral M1 protein of PROTAR viruses is E3 ubiquitin ligase-dependent.

From: Proteolysis-targeting influenza vaccine strains induce broad-spectrum immunity and in vivo protection

Extended Data Fig. 5

a, Inhibition of Cullin-RING E3 ubiquitin ligases restored the levels of PTD-tagged viral M1 proteins. HEK293T cells were transfected with constructs expressing indicated proteins for 6 h and cultured in the presence or absence of 1 μM MLN4924 for another 18 hours. Cells were harvested and subjected to detection of M1 proteins by western blotting (n = 3). b, Inhibition of E3 ubiquitin ligases by specific inhibitors restored the levels of PTD-tagged viral M1 proteins. HEK293T cells were transfected with constructs expressing indicated proteins for 48 h and cultured in the presence or absence of indicated inhibitors for another 6 hours. Cells were harvested and subjected to detection of M1 proteins by western blotting (n = 3).

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