Extended Data Fig. 9: Analysis of viral M1 antigen presentation of PROTAR vaccines.
a, Viral M1 antigen presentation of PROTAR vaccines, IIV, CAIV, and WT virus. Raw264.7 cells were infected with indicated vaccines or viruses (MOI = 0.1) for 6 hours and detected for M1 antigen presentation on the surface of Raw264.7 cells by anti-M1 antibody (left) or an anti-M1 peptide (MGLIYNRM) antibody (right) using flow cytometry. Data are shown as means ± s.d.; n = 3 biological replicates; one-way ANOVA with Dunnett’s multiple comparisons test; black P values indicate the statistical comparison to WT group; orange P values indicate the statistical comparison to IIV group; blue P values indicate the statistical comparison to CAIV group; red P values indicate the statistical comparison to M1-PTD. b, Graphs showing the relevance between PTD-mediated antigen presentation and the levels of PROTAR vaccine-induced immune responses, including IgG antibody responses (top left), IgA antibody responses (top middle and right), T cellular responses against M1 antigen in lungs (bottom left), T cellular responses against NP antigen in lungs (bottom middle), and T cellular responses against NP in spleens (bottom right) (n = 5 mice per group). The same data for M1 antigen presentation are used in all the graphs.
