Extended Data Fig. 10: Characterization of B cell responses induced by PROTAR vaccines in adult mice.
a, Graphs showing numbers of HA- or NP-binding GC B cells per 20,000 cells in spleens of C57BL/6 J mice at day 14 post-vaccination with 105 TCID50 of indicated vaccines (n = 5 mice per group). Data are shown as means ± s.d.; one-way ANOVA with Dunnett’s multiple comparisons test. b, Graphs showing numbers of HA- or NP-binding memory B cells per 20,000 cells in spleens of C57BL/6 J mice at day 14 post-vaccination with 105 TCID50 of indicated vaccines (n = 5 mice per group). Data are shown as means ± s.d.; one-way ANOVA with Dunnett’s multiple comparisons test. c, Graphs showing numbers of HA- or NP-binding plasma cells per 20,000 cells in spleens of C57BL/6 J mice at day 14 post-vaccination with 105 TCID50 of indicated vaccines (n = 5 mice per group). Data are shown as means ± s.d.; one-way ANOVA with Dunnett’s multiple comparisons test. d. Graphs showing numbers of influenza antigens (HA or NP)-specific IgG antibody-secreting plasma cells in spleens or lungs of C57BL/6 J mice at day 14 post-vaccination with 105 TCID50 of indicated vaccines (n = 5 mice per group). Data are shown as means ± s.d.; one-way ANOVA with Dunnett’s multiple comparisons test. e, Graphs showing numbers of influenza antigens (HA or NP)-specific memory B cells in spleens or lungs of C57BL/6 J mice at day 14 post-vaccination with 105 TCID50 of indicated vaccines (n = 5 mice per group). Memory B cells from mouse spleens or lungs were stimulated to proliferate and differentiate into antibody-secreting cells by R848 (1 µg/mL) and recombinant mouse (rm)IL-2 (10 ng/mL) in vitro, and levels were subsequently measured by B-cell ELISpot assay. Data are shown as means ± s.d.; one-way ANOVA with Dunnett’s multiple comparisons test. f, Immunofluorescence images showing GC formation in lungs (left) and spleens (right) of C57BL/6 J mice at day 14 post-vaccination with 105 TCID50 PTDβ-TrCP. Representative images are shown; n = 3 biological replicates. Green, GL7; Red, B220; Blue, DAPI-stained nuclei. Scale bar, 100 µm.
