Extended Data Fig. 1: Systematic investigation on the propagation competence of PROTAR viruses in MDCK-TEVp and conventional MDCK.2 cells.

MDCK-TEVp cells or MDCK.2 cells were infected with indicated viruses (MOI = 0.001) for 48 h and supernatants were collected for quantification of viral titers by TCID50 assay. The factors of decrease in viral titers of PROTAR viruses in MDCK.2 cells relative to those in MDCK-TEVp cells are indicated in the graph. The dotted line indicates the detection limit (102 TCID50/mL). Data are shown as means ± s.d. The experiments were independently performed two times.