Extended Data Fig. 6: Galactose supports A. muciniphila growth and succinate production.
(a, b) A. muciniphila (1*105 CFU) were incubated in LB medium with or without galactose (25 mM) for 48 h and the growth curve of A. muciniphila was examined by OD600 measurement (a) and qPCR (b). Each time point contains at least five replicates. (c, d) The transcriptional expression of galactosidases and galactokinase in A. muciniphila were detected by regular RT-PCR (c) and qPCR (d). Amuc_1100 was detected as positive control. Each column contains six replicates. (e) A. muciniphila were cultured in BHI medium with galactose (25 mM) or 0.05% mucin, and the production of succinate in the supernatants was quantified by LC-MS (n = 3). Each dot represents one replicate in one column. Data are representative of two independent experiments (a–e). Statistical significance was tested by ordinary two-way ANOVA with Dunnett’s multiple comparisons test (a, b) and two-sided one-way ANOVA with Tukey’s test adjusted for multiple comparisons (e). Error bars represent the mean ± SEM.
