Extended Data Fig. 7: The co-culture of sorted ILC3s and organoids.
(a) The gating strategy for sorting ILC3s from the small intestine by flow cytometry. The sorted ILC3s were further stained with anti-RORγt and anti-IL-22 antibodies. (b) Representative immunofluorescence staining for galactosylation (PNA staining, green) and DAPI (nuclear staining, blue) in organoids. The scale bars shown in 50 μm. (c) IL-22 in the supernatant of organoids with ILC3s co-cultured was detected by ELISA (n = 7). Each dot represents one biological replicate in one column (c). Data are representative of two independent experiments (b, c). Statistical significance was tested by unpaired two-sided Student’s t-test. Error bars represent the mean ± SEM.
