Extended Data Fig. 8: ILC3 regulates galactosylation by C1galt1.
(a) C1galt1 expression in the small intestine of the Id2fl/fl (n = 8) and RorcCreId2fl/fl (n = 9) mice was detected by qPCR. (b) Representative flow cytometry analysis for MC38 cell line treated with different concentrations of itraconazole (ITZ) that inhibits the function of C1GALT1 protein for 48 h (n = 3). (c) C1galt1 expression in intestinal organoids treated with or without IL-22 (5 ng/ml) at day 4 was detected by qPCR (n = 8). Each dot represents one individual mouse (a, c) or one replicate in one column (b). Data are pooled from two independent experiments (a, c). Data are representative of two independent experiments (b). Statistical significance was tested by unpaired two-sided Student’s t-test (a, c) and two-sided one-way ANOVA with Tukey’s test adjusted for multiple comparisons (b). Error bars represent the mean ± SEM.
