Fig. 2: Bacteriocin T8 is associated with growth inhibition in emergent lineage isolates.
From: Bacteriocin production facilitates nosocomial emergence of vancomycin-resistant Enterococcus faecium
a, Pairwise spot killing assay with representative ST17 (historical lineage) and ST117 (emergent lineage) isolates. The dashed circle shows where the ST17 isolate was spotted onto the ST117 lawn but did not grow. b, Pairwise spot killing assay with reference isolates from each of the 11 main lineages within the UPMC collection. The inhibition zones (mm) are shaded from highest inhibition (burgundy) to no inhibition (grey). Inhibition zone values were averaged from three biological replicates. The midpoint-rooted phylogenetic tree was constructed using RAxML with 100 bootstraps based on a single-copy core genome alignment produced by Roary. The scale bar represents nucleotide substitutions per site. c, Spot killing assay results of 28 VREfm isolates spotted onto a lawn, once per isolate, of the bacteriocin T8-negative, ST17 VRE32530 representative isolate. Isolates are grouped by presence (burgundy) or absence (grey) of bacteriocin T8 in their genomes. The P value indicates significance from a two-tailed Mann–Whitney test (unadjusted, P ≤ 0.0001). d, Abundance of bacteriocin T8 within main STs at UPMC. Emergent lineages are noted with a black star.
