Extended Data Fig. 4: ATG5 mediates protection during M. tuberculosis infection by restricting PAD4-dependent NET release.

(A-L) Flow cytometry quantification of immune cell abundance in the lungs of Mtb infected mice at 21 dpi. Each datapoint represents one biological replicate from at least 2 independent experiments. Open blue squares, Atg5^fl/fl; closed blue squares, Atg5^fl/fl-LysM-Cre; open pink squares, Padi4^−/−/Atg5^fl/fl; closed pink squares, Padi4^−/−/Atg5^fl/fl-LysM-Cre. (J) Additional representative H&E-stained lung sections from GFP-Mtb infected mice at 21 dpi. Scale bar is 500μm. (K) Additional representative merged tiled confocal immunofluorescence images at 10X magnification of lung sections from GFP-Mtb infected mice at 21 dpi that were probed with antibodies to detect citrullinated histone H3 (H3Cit; red), Ly6G (neutrophil marker; cyan), and DNA (Hoechst; blue). GFP-Mtb is shown in green. Scale bar is 1 mm. All graphs report the mean ± SD. Statistical differences were determined by or one-way ANOVA and Šídák multiple comparison test. * P < 0.05, ** P < 0.01. Differences that are not statistically significant are designated as ns. Complete statistical analysis including the number of samples used is in Supplementary Table 4.