Fig. 4: The photocycle of HeimdallR1.
From: Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
a, Chromatogram of HPLC analyses (left) and the compositions of the retinal isomers (right) (n = 3, mean ± s.d.) under the dark (DA, blue), light (green) and light-adapted (LA, orange) conditions, where AT, 13C, 11C, syn and anti indicate all-trans, 13-cis, 11-cis, syn and anti configurations, respectively. b, Two-dimensional plot of transient absorption change. The labels of positive peaks indicate the absorption increase derived from the absorptions of K, M, O intermediates, and β-band. The negative peaks labelled in HeimdallR1 represent the bleaching of the initial state. c, Transient absorption spectra at different time points of HeimdallR1 without (top), with lutein (middle) and with fucoxanthin (bottom). * indicates the absorption change of xanthophylls in c and d. d, Time course of the transient absorption change of HeimdallR1 without (top), with lutein (middle) and with fucoxanthin (bottom). e, Absorption spectra of photointermediates of HeimdallR1 without (left), with lutein (middle) and with fucoxanthin (right). * indicates the peaks derived from the absorption change of xanthophylls. f, Photocycle models of HeimdallR1 without (top), with lutein (middle) and with fucoxanthin (bottom).
