Extended Data Fig. 1: Cells with reduced levels of c-di-AMP are more sensitive to envelope-targeting antibiotics, while cells with increased levels of c-di-AMP are more resistant.
From: Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis
Representative growth curves of the indicated strains grown in LB with 5, 50 µM, or 500 µM IPTG treated with sub-inhibitory concentrations of the indicated antibiotics. (A) Red lines show cells that express low levels of CdaA with 5 µM IPTG and grey lines show cells that express wild-type levels of CdaA with 50 µM IPTG. 50 µM IPTG is closest to physiological expression levels (Extended Data Fig. 3e). (B) Blue lines show cells that express high levels of CdaA with 500 µM IPTG in the absence of the phosphodiesterases GdpP and PgpH. All experiments in this figure were performed in biological triplicate and representative growth curves are shown.
