Extended Data Fig. 1: A genome-wide optical pooled screen identifies genes affecting VP35 protein, RNA, and c-Jun.
From: Single-cell image-based screens identify host regulators of Ebola virus infection dynamics
(a) Integration of optical pooled screening workflow with RNA FISH detection using HCR amplification. (b) Histograms of intensity features in five channels for non-targeting controls cells that were infected or not infected in the genome-wide optical pooled screen at 28 h. (c) Top 40 hits with increased or decreased VP35 protein levels and the number of non-Ebola virus genetic screens or Ebola-specific genetic screens they scored in. Genes not previously associated with Ebola in the literature are marked with an orange asterisk. (d) Gene set enrichment analysis of genes with significantly decreased (purple) or increased (gold) Ebola virus VP35 protein levels. (e) Volcano plot showing genes that scored significantly for changes in VP35 RNA levels by FISH. (f) Enrichr analysis of gene ontology terms significantly enriched in genes that reduced VP35 RNA levels. (g) Volcano plot showing genes that scored significantly for changes in c-Jun levels. (h) Enrichr analysis of gene ontology terms significantly enriched in genes that reduced or enhanced c-Jun levels.
