Extended Data Fig. 2: Quality control and filtering information for genome-wide optical pooled screen.
From: Single-cell image-based screens identify host regulators of Ebola virus infection dynamics
(a) Per-channel and per-plate mean intensities across 2562 fields of view for two out of eight plates in the genome-wide screen. (b) Same as (a) but post illumination-correction. (c) Same as (b) but after averaging across all channels for each plate and removing the bottom 10th percentile of each image. (d) Scatterplots of i and j coordinates for VP35 median intensity per-cell (top) and vimentin median intensity per-cell (bottom) for 10,000 randomly selected cells across the entire genome-wide screen post post-illumination correction. (e) Frequency of i and j coordinates for cells pre-filtering for duplicate phenotyping cells and cells at field of view edges and post-filtering. (f) Pre-normalization mean per-field of view FISH median intensity (plate I, well A1) pre-normalization and post-scaling to mean and unit variance based on features for non-targeting cells within -/+ the width of a field of view of each cell of interest. (g) Performance for the random forest classification of apoptotic, mitotic, and interphase cells trained on manual annotations.
